水稻抗感纹枯病品种Ospgip1基因的表达特征

doi:10.3969/j.issn.10017216.2012.05.018

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (5): 629-632.DOI: 10.3969/j.issn.10017216.2012.05.018

水稻抗感纹枯病品种Ospgip1基因的表达特征

陈夕军^{1, 2},刘晓维¹,左示敏²,童蕴慧¹,潘学彪^2,* ,徐敬友^1,*

¹ 扬州大学园艺与植物保护学院，江苏扬州 225009； ²扬州大学江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室，江苏扬州 225009;

收稿日期:2011-09-11 修回日期:2011-12-17 出版日期:2012-09-10 发布日期:2012-09-10
通讯作者: 潘学彪2,*,徐敬友1,*
基金资助:
国家转基因生物新品种培育重大专项（2009ZX08001014B）；江苏省自然科学基金资助项目（BK2010305）。

Expression Characteristics of the Ospgip1 Gene in Rice Cultivars with Resistance or Susceptiblility to Sheath Blight

CHEN Xijun ^{1, 2} , LIU Xiaowei ¹, ZUO Shimin ², TONG Yunhui ¹, PAN Xuebiao ^2,* , XU Jingyou ^1，*

¹ Horticulture and Plant Protection College, Yangzhou University, Yangzhou 225009, China; ² Key Laboratory of Plant Functional Genomics of Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China;

Received:2011-09-11 Revised:2011-12-17 Online:2012-09-10 Published:2012-09-10
Contact: PAN Xuebiao2,*, XU Jingyou1，*

摘要/Abstract

摘要： 从水稻基因组DNA中扩增出1 184 bp的Ospgip1基因片段。该片段包含930 bp的完整开放阅读框，终止密码子为TAA，无内含子。RTPCR结果表明，Ospgip1基因在水稻抗、感纹枯病品种中均能表达，但不同生育期、不同部位表达量有差异。实时PCR结果显示，抗病品种YSBR1和中感品种Jasmine 85中Ospgip1的表达量要明显高于感病品种Lemont；稻苗黄化处理后，无论是抗病品种还是感病品种的Ospgip1表达量均显著提高；纹枯病菌侵染使得抗病品种Ospgip1表达量大大增加，而对感病品种影响不大。

关键词: 表达特征, 水稻品种, Ospgip1基因, 抗性

Abstract: A fragment of 1 184 bp of the Ospgip1 gene including a complete open reading frame (OFR) of 930 bp had been amplified, which had the termination codon of TAA and no intron. RTPCR results showed that the Ospgip1 gene could be expressed in all the rice cultivars while their expression levels differed among growth stages or organs. Realtime PCR results indicated that the expression levels in resistant cultivars YSBR1 and Jasmine 85 were significantly higher than that in the susceptible cultivar Lemont. The expression level was also upregulated in the etiolated seedlings. Inoculation with the pathogen of rice sheath blight significantly upregulated the expression level of the Ospgip1 gene in resistant cultivars while exerted no impact on the expression level of the gene in the susceptible one.

Key words: expression characteristics, rice cultivar, Ospgip1 gene, resistance

中图分类号:

陈夕军1, 2,刘晓维1,左示敏2,童蕴慧1,潘学彪2,*,徐敬友1,*. 水稻抗感纹枯病品种Ospgip1基因的表达特征[J]. 中国水稻科学, 2012, 26(5): 629-632.

CHEN Xijun1, 2, LIU Xiaowei1, ZUO Shimin2, TONG Yunhui1, PAN Xuebiao2,*, XU Jingyou1，*. Expression Characteristics of the Ospgip1 Gene in Rice Cultivars with Resistance or Susceptiblility to Sheath Blight[J]. Chinese Journal of Rice Science, 2012, 26(5): 629-632.

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水稻抗感纹枯病品种Ospgip1基因的表达特征

Expression Characteristics of the Ospgip1 Gene in Rice Cultivars with Resistance or Susceptiblility to Sheath Blight

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